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Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits


Analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) using these complete reagent kits.

Manufacturer: thermo scientific™  89983

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Catalog No. PI89983

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Description & Specifications

Specifications

Kit Contents DMEM Media for SILAC, 2 x 500mL
Description SILAC Protein Quantitation Kit - DMEM
Formulation Multi-component kit with heavy and light L-Lysine and DMEM Media
Quantity 1 use kit
For Use With (Application) SILAC experiments (stable isotope labeling with amino acids in cell culture)
Sufficient For One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)

Thermo Scientific™ SILAC Protein Quantitation Kits are used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Reproducible – eliminates intra-experimental variability caused by differential sample preparation

  • Flexible – media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling

  • Compatible – label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM, RPMI-1640 or DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others

  • Optimized, specialized media – the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High-quality supplements – heavy amino acids with >99% isotope purity; the Stem Cell Screened Dialyzed FBS and Serum Substitute for Mouse Embryonic Stem Cells are tested to ensure that they do not promote stem cell differentiation

Recommended for:

Quantitative analysis of relative changes in protein abundance from different cell treatments; Quantitative analysis of proteins for which there are no antibodies available; Protein expression profiling of normal vs. disease cells; Identification and quantification of hundreds to thousands of proteins in a single experiment