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Thermo Scientific™ Pierce™ SILAC Media and Sera

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

$68.51 - $518.70

Products
Catalog Number Mfr. No. Formulation Quantity Price Quantity    

PI88420

 
thermo scientific™
88420
DMEM media minus L-Lysine and L-Arginine 6 x 500mL Each for $322.35

PI88421

 
thermo scientific™
88421
RPMI-1640 media minus L-Lysine and L-Arginine 6 x 500mL Pack of 6 Each for $322.35

PI88422

 
thermo scientific™
88422
MEM media minus L-Lysine and L-Arginine 500mL Each for $116.55

PI88423

 
thermo scientific™
88423
IMDM media minus L-Lysine and L-Arginine 500mL Each for $117.60

PI88424

 
thermo scientific™
88424
Ham's F12 media minus L-Lysine and L-Arginine 500mL Each for $116.55

PI88425

 
thermo scientific™
88425
DMEM media minus L-Leucine, L-Lysine and L-Arginine 135g Each for $518.70

PI88426

 
thermo scientific™
88426
RPMI media minus L-Leucine, L-Lysine and L-Arginine 104g Each for $518.70

PI88441

 
thermo scientific™
88441
McCoy's 5A media minus L-Lysine and L-Arginine 500mL Each for $116.55

PI89984

 
thermo scientific™
89984
RPMI-1640 media minus L-Lysine and L-Arginine 500mL Each for $68.51

PI89985

 
thermo scientific™
89985
DMEM media minus L-Lysine and L-Arginine 500mL Each for $68.51
Description & Specifications

These Thermo Scientific Pierce cell culture media and serum components are optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

  • Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

  • Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation