Thermo Scientific™ Pierce™ Secondary Antibody Coated Plates
Perform binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.
Manufacturer: Thermo Scientific™ 15334
Thermo Scientific™ Pierce Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.
These plates are pre-coated with goat anti-mouse (GAM) or goat anti-rabbit (GAR) IgG polyclonal antibodies, allowing assay-specific antibodies to be captured even from impure protein samples. The plates are supplied pre-blocked and ready-to-use. Pierce Secondary Antibody Coated Plates can be used for direct, indirect, competitive or sandwich assays and are available in clear for colorimetric assays, white for chemiluminescent assays and black for fluorescent assays.
- Prevents antibody denaturation caused by direct binding
- Unlike Protein A or G, these bind only to target species
- Antibody-binding capacity is higher than direct adsorption
- Pre-blocked to reduce nonspecific binding
- Less expensive than Protein A or G plates
|Binding approx. 7pmol mouse IgG per well|
|Goat Anti-Mouse IgG Antibody|
|Black, 96-well plates, coated with 100uL of Goat Anti-Mouse IgG Antibody and blocked with 200uL of SuperBlock Blocking Buffer|