Thermo Scientific™ Pierce™ S-Nitrosylation Western Blot Kit
Perform sensitive detection of protein S-nitrosocysteine post-translational modifications with a complete, easy-to-use kit.
Manufacturer: Thermo Scientific™ 90106
Perform sensitive detection of protein S-nitrosocysteine post-translational modifications with the complete, easy-to-use Thermo Scientific™ Pierce S-Nitrosylation Western Blot Kit.
Specific – optimized labeling reactions switch S–nitrosocysteine post translational modifications with iodoTMT Reagent for easy detection by Western blot using anti–TMT antibody
Better signal-to-noise – uses a non–biological iodoTMT Reagent for labeling instead of HPDP–biotin, resulting in less background during Western blot detection
Workflow-compatible – labeling method is compatible with alternative methods of analysis, including enrichment via anti–TMT resin and multiplex quantitation by mass spectrometry
Convenient – all needed reagents included one kit
Cysteine–blocking reagent, reducing agent, reaction buffer, iodoTMT™ labeling reagent, and anti-TMT antibody
|Detection of the TMT label in Western blots|
|100mM HEPES, pH 7.8; 1mM EDTA, 0.1mM neocuproine, 1% SDS|