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Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Manufacturer:  Thermo Scientific™ 89901

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Catalog No. PI89901


 

Specifications

Description

Thermo Scientific™ RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.

Highlights:

  • Convenient – ready-to-use solution

  • Flexible – compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification

  • Versatile – enables extraction of cytoplasmic, membrane and nuclear proteins

  • Disclosed formulation – contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues

Description & Specifications

Specifications

Pierce RIPA Buffer
250mL
Liquid
25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS
Approx. 5 million cells per 1mL of reagent
Cultured mammalian cells
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