Thermo Scientific™ Pierce Rapid Mouse Antibody Isotyping Kits are single-use, cassette-based kits for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.The kits are composed of small cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kits determine mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM.
The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.
Long shelf life – stable for more than 1 year at room temperature
Single-step – add diluted antibody sample to the loading-well of cassette
Fast – within 5 minutes a band appears that indicates the antibody isotype
Sensitive – detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL
Specific – assay does not crossreact with fetal bovine serum (FBS)
Reliable – results are similar to the standard ELISA-based isotyping assays
Ten pouches, each containing two (Part No. 26178) or three (Part No. 26179) cassettes, plus a bottle of sample diluent
|10 test kit|
|Isotyping Cassettes (three per pouch), 10 pouches|
|10 isotype determinations|