Recombinant Protein L, covalently attached to a blocked magnetic bead surface, selectively binds mouse and human antibodies through kappa light chains. The beads are commonly used to purify monoclonal antibodies in cell culture supernantants supplemented with bovine serum as Protein L does not bind bovine IgG. The Pierce Protein L Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high-throughput workflows.
Selective – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
Low non-specific binding – stable, pre-blocked beads provide clean purification of antibody
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific™ KingFisher Instruments)
Purification of monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G magnetic beads
Purification of monoclonal antibodies from culture supernatants supplemented with bovine serum
Protein L IP and co-IP assays
Purification of ScFv and Fab fragments containing kappa light chains