Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers
Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.
Manufacturer: Thermo Scientific™ 37585
Thermo Scientific™ Pierce Protein-Free Blocking Buffers are PBS and TBS formulations of a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.Traditional blocking buffers contain proteins that can cross-react with immunodetection systems, resulting in high background and reduced signal. Typical commercial alternatives to protein-based blocking agents are ineffective. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes and other surfaces for ELISA, Western blotting, glass slide arrays and other applications.
Protein-free blocker – minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Application-compatible – effective in all kinds of protein detection systems, including Western blots (membranes), ELISA (microplates) and arrays (coated glass slides)
Streptavidin-friendly – absolutely free of biotin; no possible interference with avidin-biotin detection systems
High-performance – optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
Convenient – supplied as ready-to-use 1X formulations in TBS and PBS with and without 0.05% Tween™-20
|ELISA, IHC, WB|
|Proprietary compound in Tris-buffered saline, pH 7.4 with Kathon antimicrobial agent|
|10 mini blots at 10mL each|
|Protein-Free (TBS) Blocking Buffer|