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Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.

Manufacturer:  Thermo Scientific™ 37572

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Catalog No. PI37572



Thermo Scientific™ Pierce Protein-Free Blocking Buffers are PBS and TBS formulations of a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

Traditional blocking buffers contain proteins that can cross-react with immunodetection systems, resulting in high background and reduced signal. Typical commercial alternatives to protein-based blocking agents are ineffective. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes and other surfaces for ELISA, Western blotting, glass slide arrays and other applications.


  • Protein-free blocker – minimizes or eliminates cross-reactivity associated with protein-based blocking buffers

  • Application-compatible – effective in all kinds of protein detection systems, including Western blots (membranes), ELISA (microplates) and arrays (coated glass slides)

  • Streptavidin-friendly – absolutely free of biotin; no possible interference with avidin-biotin detection systems

  • High-performance – optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

  • Convenient – supplied as ready-to-use 1X formulations in TBS and PBS with and without 0.05% Tween™-20



Proprietary compound in Phosphate-buffered saline, pH 7.4, with Kathon preservative
30 blots or microplates at 30mL each
Protein-Free (PBS) Blocking Buffer