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Thermo Scientific™ Pierce™ Protein AG Magnetic Beads


Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.

Manufacturer: thermo scientific™  88803

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Catalog No. PI88803

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Description & Specifications

Specifications

Description Pierce Protein A/G Magnetic Beads
Formulation Magnetite- and protein-coated polymer beads at 10mg/mL in water with sodium azide
Quantity 5mL
Sufficient For Binding 55 to 85ug rabbit IgG/mg beads
Support Type Magnetic Bead

The Thermo Scientific™ Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G. This enables capture of antibodies from a wider range of species and isotypes than either protein alone. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample. These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher™ Instruments.

Highlights:

  • High capacity – nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment

  • Low non-specific binding – stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix)

  • Flexibility – convenience of IgG binding domains of both Protein A and Protein G on one bead

  • Compatibility - beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments)

  • Assay consistency – magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes

Properties of Thermo Scientific Pierce Protein A/G Magnetic Beads:

  • Composition: Magnetite-coated polymeric beads blocked and covalently coated with a monolayer of recombinant Protein A/G
  • Diameter: 1μm
  • Concentration: 10mg/mL
  • Binding Capacity: 55-85μg rabbit IgG per mg magnetic particles

Requires:

Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device

Recommended for:

IP and Co-IP experiments (see complete kit); Immunoprecipitation for analysis in non-reducing conditions; Antibody purification