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Thermo Scientific™ Pierce™ Protein A, G, and L Coated Plates


Capture antibody complexes with these alternatives to direct, passive adsorption for immobilizing antibodies for ELISA and other plate-based assays.

Manufacturer: thermo scientific™  15155

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Catalog No. PI15155

  • / Pack of 5 Each
 

Description & Specifications

Specifications

Binding Target IgG
Color Black
Formulation Black, 96-well plates, coated with 100uL of Protein A and blocked with 200uL of SuperBlock Blocking Buffer
Format 96-Well
Quantity 5 plates
Sufficient For Binding approx. 4pmol rabbit IgG per well
Surface Coating Protein A

Thermo Scientific™ Pierce Protein A, G, A/G and L Coated Plates provide alternatives to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G or Protein L). They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Highlights:

  • Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
  • Orient antibodies for maximum antigen-binding capacity
  • Immobilize antibodies for plate assays without prior purification
  • Ensure minimal variation (<5% well-to-well) from consistent coating
  • Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
  • Bind 2 to 5pmol of antibody per well (based on tests with rabbit IgG)

Protein A/G Coated Microplates:

  • Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
  • Binds to most IgG species because it combines the specificities of Protein A and Protein G
  • Binds to Fc region of antibodies, ensuring optimal orientation
  • Binds well using a wide range of pH

Protein A Coated Microplates:

  • Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
  • Binds strongly to mouse IgG2a, IgG2b and IgG3
  • Binds to Fc region of antibodies for optimal orientation

Protein G Coated Microplates:

  • Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
  • Binds only to IgG—no cross-reactivity with other antibody classes
  • Binds to Fc region of antibodies for optimal orientation

Protein L Coated Microplates:

  • Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
  • Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
  • Binds to Fab fragments and ScFv
  • Does not bind bovine, goat or sheep immunoglobulin
  • Binds weakly to rabbit immunoglobulin
  • Pre-blocked to reduce nonspecific binding