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Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry


Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Manufacturer: thermo scientific™  90051

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Catalog No. PI90051

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Description & Specifications

Specifications

Description Pierce Lys-C Endoproteinase, MS Grade
Formulation Lyophilized enzyme powder
MS-Compatible Yes
Quantity 1 x 20µg
Sufficient For Lot-specific activity

These Thermo Scientific™ Pierce Proteases are small, convenient packages of individual, purified highly-specific proteases for use in various combinations to obtain better sequence coverage and protein identification for mass spectrometry.

Improvement in sequence coverage and overall protein characterization can result from improving peptide chromatographic or ionization properties. The masses of these larger peptides can be accurately measured at high charge states on popular high-resolution mass spectrometers, reducing the database search time and increasing the sequence coverage and confidence. Finally, larger peptides can be fragmented effectively by new fragmentation techniques like electron transfer dissociation (ETD) or electron capture dissociation (ECD) that leave post-translational modifications, like phosphorylation, intact.

Highlights:

  • Proteases offer specific cleavage at multiple sites, enabling exploration of primary protein structure
  • Better protein characterization results from overlapping peptides with complementary chromatographic, ionization and fragmentation properties
  • Protocols are designed for MS applications
  • Streamlined data processing and analysis improves interpretation and confidence