Thermo Scientific™ Pierce™ Prestained Protein Molecular Weight Marker
A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
Manufacturer: Thermo Scientific™ 26612
Thermo Scientific™ Prestained Protein Molecular Weight Marker is a mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents.
Size range – six proteins spanning 20 to 120kDa
Ready-to-use – supplied in a loading buffer for direct loading on gels
Prestained – for direct viewing during electrophoresis
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Dye-stained proteins in 62.5mM Tris-HCl (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1.5mM NaN3 and 33% glycerol; Component proteins: beta-galactosidase (120kDa), bovine serum albumin (85kDa), ovalbumin (50kDa), cardonic anhydrase (35kDa), beta-lactoglobulin (25kDa), lysozyme (20kDa)
Monitoring protein migration during SDS-polyacrylamide gel electrophoresis; Monitoring protein transfer onto membranes after Western blotting; Sizing of proteins on SDS-polyacrylamide gels and Western blots
|Pierce Prestained Protein Molecular Weight Marker|
|6 proteins from 20 to 120kDa in reducing sample buffer|
|50 to 100 mini gel lanes at 5 to 10μL per lane|