Thermo Scientific™ Pierce™ PNPP Substrates
Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.
Manufacturer: Thermo Scientific™ PI37620
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.
- Absorbance maximum of 505nm for measurement by ELISA plate readers
- Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
- Safer to use – solution contains a non-mercury, non-azide preservative
|Two-component kit with PNPP tablets and 5X substrate buffer|
|Pierce PNPP Substrate Kit|
|Preparing 525mL of substrate (5mL with each tablet)|
|Diethanolamine Buffer, 225mL|