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Thermo Scientific™ Pierce™ PES Protein Concentrators


Concentrate and desalt biological samples and perform buffer exchange with disposable polyethersulfone (PES)-membrane ultrafiltration centrifugal devices.

Manufacturer: thermo scientific™  88513

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Catalog No. PI88513

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Description & Specifications

Specifications

Description Protein Concentrators PES, 10K MWCO, 0.5mL
Formulation Polyethersulfone membrane bonded to polycarbonate tube
Quantity 25 devices
Sufficient For 25 samples, each 0.1 to 0.5mL
MWCO 10000 Da
Capacity (Metric) 0.5mL
Item Description 10K MWCO; 25 units

The Thermo Scientific™ Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples with volumes of 0.5mL or less.

Pierce Protein Concentrators PES can be used for processing sample volumes up to 0.5mL and are available in four distinct molecular-weight cutoffs (MWCOs) of 3K, 10K, 30K and 100K. For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500μL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes. The 0.5mL Pierce Concentrators, PES are compatible with most bench-top microcentrifuges with fixed-angle rotors that accommodate 2.2mL tubes.

Highlights:

  • Rapid protein concentration – sample concentration of 10- to 30-fold can be achieved in 10 minutes or less

  • Excellent protein recovery – unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions

  • Instrument-compatible – use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning

  • Versatile – easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

Recommended for:

Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations; Concentration of protein peaks following gel-permeation chromatography; Removal of unincorporated label following protein modification reactions; Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns; Separating large molecules from small molecules.