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Thermo Scientific™ Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers.OPD (o-phenylenediamine dihydrochloride) yields a water-soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492nm. OPD can easily be dissolved in a substrate buffer such as Thermo Scientific Stable Peroxide Substrate Buffer (Part No. 34062).
- Water soluble product when reacted with peroxidase
- Absorbance maximum of 492nm