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Normal Goat Serum Control, Invitrogen™


Normal Goat Serum Control, 31873, from Invitrogen™., 31873, from Invitrogen™. Species Reactivity: Not Applicable; Applications: Blocking Assay, Control, ICC, IF, IHC.

Manufacturer: pierce biotechnology inc  PI31873

Catalog No. PI31873

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Description & Specifications

Specifications

Antigen Normal Goat Serum
Applications Blocking Assay
Applications Control
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry
Conjugate Unlabeled
Format Serum
Formulation 600mg unpurified control antibody in whole serum diluted in PBS and 0.05% sodium azide; pH 7.6
Gene Alias Negative Control, Control
Host Species Goat
Quantity 600mg

Normal Goat Serum Control, 31873, from Invitrogen., 31873, from Invitrogen. Species Reactivity: Not Applicable; Applications: Blocking Assay, Control, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.

Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates, making it useful for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components.
Host Species: Goat
Formulation: 600mg unpurified control antibody in whole serum diluted in PBS and 0.05% sodium azide; pH 7.6
Synonyms: Negative Control, Control
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum. Restoration and Storage: Store product at 4°C until opened. Restore with 10.0mL distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, freeze rehydrated undiluted product in small aliquots at 20ºC. Reconstitute with 10.0mL of distilled water. Country of Origin: USA
References:
Test: BLOCK, Species: Hu, Reference: A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients. (Dec 2013) Springerplus, Vol: 2, 1, 263, Kragstrup TW,Vorup-Jensen T,Deleuran B,Hvid M; Pubmed ID: 23875127