Thermo Scientific™ Pierce™ Nickel Coated Plates
Bind His-tagged fusion proteins for ELISA and other plate-based assays to study interactions of expressed recombinant proteins.
Manufacturer: Thermo Scientific™ PI15142
Thermo Scientific™ Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.
- Detergents used to lyse cells do not inhibit binding to Ni2+coated plates as they do with plain polystyrene
- The detection limit is 1ng of histidine fusion protein
- Better binding for more sensitive assays compared to other commercially available nickel-activated plates
|Pierce Nickel Coated Plates, Clear, 96-Well Strip|
|Binding approx. 9pmol His-tagged protein per well|
|Clear, 96-well strip plates, coated with 200uL of Nickel ion and blocked with 200uL of BSA|