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Thermo Fisher Scientific™ Pierce™ Ni-NTA Magnetic Agarose

Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins

Manufacturer:  Thermo Fisher Scientific™ PI78605

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Catalog No. PI78605


 

Specifications

Description

Magnetic Agarose, Ni-NTA; Thermo Fisher Scientific; Pierce; Protein Purification Resins; Affinity Separation Particles; 4mL

Product features:
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.
  • High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
  • High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
  • Low nonspecific binding— optimized purification protocol results in better tagged-protein purification
  • Versatile—purify proteins using native or denaturing conditions
  • Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives
Applications:
  • Protein purification and isolation
  • High throughput protein purification using automated magnetic particle processor
Target molecule: His-Tag
Description & Specifications

Specifications

Ni-NTA
4mL
Store at 4°C
Magnetic Agarose
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