Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells
Perform preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis with this easy-to-use, comprehensive kit.
Manufacturer: Thermo Scientific™ 84840
Perform preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis with Thermo Scientific™ Pierce Mass Spec Sample Prep Kit for Cultured Cells, an easy-to-use, comprehensive kit.
Complete – includes all reagents, a digestion indicator control, proteases and an optimized protocol needed to process up to 20 samples
Simple – user friendly kit can provide reproducible results even for non-expert MS analysts
Flexible – can be adapted to handle sample sizes between 10 to 200μg
High yield – total protein yield from 1 million cells is greater than 100μg
Optimized – cysteine reduction and alkylation are 100% with less than 1% over alkylation of non-cysteine residues
Efficient - percentage of missed cleavages is less than 10%
Compatible – final preparation is ready for direct MS analysis and other downstream applications, including mass-tag labeling
Kit contains proprietary lysis and digestion buffers, digestion indicator, DTT, iodoacetamide, Lys-C, trypsin
|Pierce Mass Spec Sample Prep Kit for Cultured Cells|
|Trypsin Protease, MS Grade, 2 x 20µg, Trypsin Storage Solution, 250µL, IAA, No-Weigh Format, 24 tubes, DTT, No-Weigh Format, 24 tubes, Digestion Buffer, 5mL, Pierce Digestion Indicator, 10µg, Lys-C Protease, MS Grade, 20µg, Cell Lysis Buffer, 5mL|
|20 protease digestions and preparations from 100µg of cell lysate|
|20 rxn kit|