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Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells


Perform preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis with this easy-to-use, comprehensive kit.

Manufacturer: thermo scientific™  84840

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Catalog No. PI84840

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Description & Specifications

Specifications

Description Pierce Mass Spec Sample Prep Kit for Cultured Cells
Formulation Multi-component kit
Kit Contents Cell Lysis Buffer, 5mL
Quantity 20 rxn kit
Sufficient For 20 protease digestions and preparations from 100µg of cell lysate

Perform preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis with Thermo Scientific™ Pierce Mass Spec Sample Prep Kit for Cultured Cells, an easy-to-use, comprehensive kit.


Additional Information and Online Ordering »

The Pierce Mass Spec Sample Prep Kit for Cultured Cells contains all the necessary reagents and enzymes to prepare up to 20 samples (1 million cells each) for MS analysis. The simple and robust workflow uses a lysis protocol that generates approximately 100μg of protein per sample and includes a control digestion indicator to monitor the efficiency of the two-step enzymatic digestion protocol. The kit procedure, which includes a Digestion Indicator as an internal protein/peptide control, produces consistently and reproducibly clean peptide mixtures for protein identification.

Highlights:

  • Complete – includes all reagents, a digestion indicator control, proteases and an optimized protocol needed to process up to 20 samples

  • Simple – user friendly kit can provide reproducible results even for non-expert MS analysts

  • Flexible – can be adapted to handle sample sizes between 10 to 200μg

  • High yield – total protein yield from 1 million cells is greater than 100μg

  • Optimized – cysteine reduction and alkylation are 100% with less than 1% over alkylation of non-cysteine residues

  • Efficient - percentage of missed cleavages is less than 10%

  • Compatible – final preparation is ready for direct MS analysis and other downstream applications, including mass-tag labeling

Includes:

Kit contains proprietary lysis and digestion buffers, digestion indicator, DTT, iodoacetamide, Lys-C, trypsin