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Thermo Scientific™ Pierce™ Maleic Anhydride (Amine-binding) Plates

Bind amine-containing peptides and other molecules to microplate wells for use in binding assays with these maleic anhydride-activated plates.

$252.00 - $1,092.00

Specifications

Binding Target Amine
Sufficient For Binding approx. 125pmol biotin-pentylamine per well
View More Specs
Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

PI15100

 
thermo scientific™
PI15100
Pierce Maleic Anhydride Activated Plates, Clear, 96-Well Strip 5 plates Pack of 5 Each for $254.10

PI15108

 
thermo scientific™
15108
Pierce Maleic Anhydride Activated Plates, White, 96-Well 5 plates Pack of 5 Each for $268.80

PI15110

 
thermo scientific™
PI15110
Pierce Maleic Anhydride Activated Plates, Clear, 96-Well 5 plates Pack of 5 Each for $252.00

PI15112

 
thermo scientific™
PI15112
Pierce Maleic Anhydride Activated Plates, Clear, 96-Well 5 x 5 plates Pack of 25 Each for $1,092.00
Description & Specifications

Specifications

Binding Target Amine
Sufficient For Binding approx. 125pmol biotin-pentylamine per well

Thermo Scientific™ Pierce Maleic Anhydride Activated Plates allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.

Pierce Maleic Anhydride Activated Plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (–NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours. Therefore, coating reactions are best performed at pH 8 to 9, and the reacted plates are best used at pH greater than 7 for ELISA and other methods.

Highlights:

  • Spontaneously react with primary amines
  • A direct, simple approach to covalently attach molecules to the well surfaces of assay plates
  • Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions