Thermo Scientific™ Pierce™ Maleic Anhydride (Amine-binding) Plates
Bind amine-containing peptides and other molecules to microplate wells for use in binding assays with these maleic anhydride-activated plates.
Manufacturer: Thermo Scientific™ PI15112
Thermo Scientific™ Pierce Maleic Anhydride Activated Plates allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.Pierce Maleic Anhydride Activated Plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (–NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours. Therefore, coating reactions are best performed at pH 8 to 9, and the reacted plates are best used at pH greater than 7 for ELISA and other methods.
- Spontaneously react with primary amines
- A direct, simple approach to covalently attach molecules to the well surfaces of assay plates
- Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions
|Pierce Maleic Anhydride Activated Plates, Clear, 96-Well|
|5 x 5 plates|
|Binding approx. 125pmol biotin-pentylamine per well|
|Clear, 96-well plates, coated with 200uL of Maleic Anhydride and blocked with n/auL of n/a|