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Thermo Scientific™ Pierce™ Iodoacetic Acid


Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Manufacturer: thermo scientific™  90034

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Catalog No. PI90034

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Description & Specifications

Specifications

Chemical Reactivity Sulfhydryl
Description Iodoacetamide, Single-Use
Formulation White solid of 2-iodoacetamide (IAM) in foil-sealed black microtubes (3 x 8 format)
Molecular Weight 184.96
Quantity 24 x 9.3mg
Sufficient For 24 separate uses for alkylation before mass spectrometry, each with up to 75 protein samples

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Iodoacetic Acid (IAA) can react with several protein functional groups but is typically used for specific S-carboxymethylation of sulfhydryls (reduced cysteines).

Iodoacetic acid reacts with sulfhydryls on cysteines, imidazolyl side chain nitrogens of histidines, the thioether of methionine and the primary amine group of lysines.The rate of reaction and specificity is dependent on the ionization level, which can be manipulated by the pH of the reaction condition.

Highlights:

  • React at slightly alkaline pH for specific S-carboxymethylation of free sulfhydryls
  • React at low pH for specific carboxymethylation of methionines
  • React at high pH to favor carboxymethylation of histidines and lysines
  • Methylate reduced cysteine peptide fragments in protease digests for mass spectrometry