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Thermo Scientific™ Pierce™ IgM Purification Kit

Easily purify IgM from mouse ascites fluid using immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

$118.65 - $1,757.70

Products
Catalog Number Mfr. No. Description Price Quantity    

PI21017

 
thermo scientific™
PI21017
Pierce IgM Elution Buffer Each for $118.65

PI22212

 
thermo scientific™
PI22212
Pierce Mannan Binding Protein Agarose Each for $1,757.70

PI44897

 
thermo scientific™
PI44897
Pierce IgM Purification Kit Each for $1,171.80
Description & Specifications

Thermo Scientific™ Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose.

Highlights:

  • Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum

  • Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use

  • Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies

  • Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity

Includes:

Kit contains a 5mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender