Thermo Scientific™ Pierce™ High Binding NeutrAvidin™ Coated Plates
Attain higher biotin-binding efficiency with these pre-blocked, ready-to-use high-capacity NeutrAvidin protein-coated microplates.
Manufacturer: Thermo Scientific™ PI15507
Thermo Scientific™ Pierce NeutrAvidin High Binding Capacity (HBC) Coated Plates are pre-blocked and ready-to-use polystyrene microplates for assays involving biotinylated antibodies or biotin-labeled molecules.
Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Thermo Scientific NeutrAvidin™ Protein is avidin that has been deglycosylated to prevent nonspecific lectin-binding. NeutrAvidin Protein has a near-neutral pI (6.3), resulting in lower nonspecific interactions in certain applications compared with streptavidin. NeutrAvidin Protein has an additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells. Pierce NeutrAvidin High Binding Capacity (HBC) Coated Plates are available in clear, white and black plates for use with colorimetric, chemiluminescent and fluorescent detection methods, respectively.
Exclusive plate-coating technology – approx. twice the capacity of typical biotin-binding plates
Robust and sensitive – NeutrAvidin Protein yields less nonspecific binding for improved signal-to-noise ratios
Broad dynamic range – extends the quantitative range so there is no need for dilutions
Save time – pre-blocked plates (two types available) reduces the number of assay steps
Flexible assay formats – choose 96- and 384-well formats and in different colors
NeutrAvidin Protein – performance equals or exceeds streptavidin but costs less
|Pierce NeutrAvidin Coated High Capacity Plates, Clear, 96-Well|
|Clear, 96-well plates, coated with 100uL of NeutrAvidin Protein and blocked with 200uL of SuperBlock Blocking Buffer|
|Binding approx. 60pmol biotin per well|