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Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.

Manufacturer:  Thermo Scientific™ 0016174

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Catalog No. PI16174



The Thermo Scientific™ Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates. Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate.


  • Highly sensitive detection of firefly luciferase activity
  • Use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal)
  • Working solution stable for up to 2 months at -20°C
  • Assay reagents compatible with other firefly luciferases
  • Amenable to automation
  • Contains a universal cell lysis buffer and optimized flash assay reagent
  • Safe non-radioactive assay


Cell lysis buffer, reaction buffer and substrate


Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies



Assay Kit
100 Reaction Kit
Reporter Enzyme, Luciferase Reporter Assay
384-well plate, 96-well plate
Chemical Substrate
Luciferase Substrate
Luciferase, Firefly Luciferase
• Firefly Flash Assay Buffer, 5 mL, store at 4°C
• D-Luciferin, Lyophilized, 3 mg, store at 4°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature

Upon receipt store kit at 4°C or store individual components as indicated above.
Mammalian Cells
Luminometer (Microplate)
Enzyme Labeled
Enhanced Chemiluminescence
Product Certifications