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Thermo Scientific™ Pierce™ Fast Western Blot Kits, ECL

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

Manufacturer:  Thermo Scientific™ 35050*

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Catalog No. PI35050



Thermo Scientific™ Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes.

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.


  • Fast – all the sensitivity of Pierce ECL substrate; saves three to four hours over traditional blotting methods

  • Convenient – no expensive hardware or extra bench space needed; eliminates membrane clogging issues

  • Economical – costs about the same as a classical Western blot; no expensive consumables or extra equipment

  • Easy – optimized kits includes all reagents except primary antibody

  • Versatile – kits work with both rabbit and mouse primary antibodies


Kits contain all reagents to perform Western blots probed with mouse and rabbit primary antibodies, including: Fast Western Antibody Diluent; Fast Western 10X Wash Buffer; Fast Western Optimized HRP Reagent; Pierce ECL Detection Reagent 1; Pierce ECL Detection Reagent 2



Pierce ECL Detection Reagent 1, 125mL, Fast Western Antibody Diluent, 500mL, Fast Western 10X Wash Buffer, 250mL, Pierce ECL Detection Reagent 2, 125mL, Fast Western Optimized HRP Reagent, 25mL
25 mini blots probed with mouse or rabbit primary antibodies
Pierce Fast Western Blot Kit, ECL Substrate
Multi-component kit
1L kit
Kit is stable for one year if reagents are stored at 4°C.