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Thermo Scientific™ Pierce™ Factor Xa


Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.

Manufacturer: thermo scientific™  PI32520

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Catalog No. PI32520

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Description & Specifications

Specifications

Description Pierce Factor Xa
Formulation At least 1mg/mL enzyme in MES-buffered saline pH 6 with 1mM benzamidine
MS-Compatible No
Quantity 250µg
Sufficient For At least 250 units of activity/mg
Storage Requirements -70°C

Thermo Scientific™ Pierce Factor Xa is a serine endopeptidase composed of two disulfide-linked subunits, whose specificity makes it particular useful in cleaving 6xHis tags from engineered His-tagged fusion proteins.

Cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg and will occasionally cleave at other basic residues. However, it will not cleave at a site followed by proline or arginine. Often used to remove fusion tags, such as the common histidine tag, from expressed proteins.

Highlights:

  • Extremely specific proteolytic activity - useful in the genetic engineering of proteins
  • Cleaves after the last amino acid in the sequence Ile-Glu-Gly-Arg
  • Activity is >130U/mg using a synthetic Ile-Glu-Gly-Arg peptide with p-nitroanilide derivatized arginine as the substrate; each unit of enzyme cleaves 1mol/minute of substrate at 37°C in 5mM MES, 0.5M NaCl, 1mM benzamidine at pH 6 with 1% BSA

Additional Features:

  • Source: Bovine Plasma
  • Activity: 125units/mg
  • Supplied: ≥5mM MES, pH 6, 0.5M NaCl
  • Protein Concentration: 1-2mg/mL

Ordering Information:

Supplied as frozen liquid.