Thermo Scientific™ Pierce™ EGFR In-Cell ELISA Kit
Measure total and phosphorylated epidermal growth factor receptor (phospho-EGFR) using EGFR antibodies detected by fluorescence or TMB substrate in whole-cell plate assays.
Manufacturer: Thermo Scientific™ 62205
Thermo Scientific™ Pierce EGFR In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure total and phosphorylated epidermal growth factor receptor (phospho-EGFR) in whole cells.The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
|Pierce EGFR Colorimetric In-Cell ELISA Kit|
|Blocking Buffer, Components included only in the target-specific kits:Antibody #1 and Antibody #2, Elution Buffer, 2 x 30mL, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, HRP Conjugate, TMB Substrate, TMB Stop Solution, Janus Green Whole-Cell Stain, Elution Buffer, Thin Plate Seal Assembly|
|96 test kit|
|1 hr 20 min|
|Multi-component kit with HRP-conjugate|
|Colorimetric Microplate Reader|
|Supernatants (10,000 cells)|
|96 microplate wells of overnight-cultured cells|