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Thermo Scientific™ Pierce™ Dextran Desalting Columns


Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with crosslinked dextran-based gel-filtration columns.

Manufacturer: thermo scientific™  PI43233

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Catalog No. PI43233

  • / Pack of 5 Each
 

Description & Specifications

Specifications

Description Dextran Desalting Columns, 5K MWCO, 10mL
Formulation Polypropylene columns with dextran resin
Quantity 5 columns
Sufficient For 5 samples, each 0.5 to 2.5mL
MWCO 5000 Da
Capacity (Metric) 2.5mL

Thermo Scientific™ Pierce Dextran Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low molecular-weight buffer salts and reagents.

Dextran Desalting Columns are pre-packed with crosslinked dextran beads that have a wet bead diameter of 50 to 150μm and a molecular-weight cutoff (MWCO) or exclusion limit equal to 5000. The two sizes of pre-packed columns are appropriate for processing samples ranging from 0.25 to 2.5mL. The columns desalt or buffer-exchange biological samples that are added to the top of the column and allowed to drip through the resin bed. Dissolved salts and other small molecules migrate through the column more slowly than proteins and other macromolecules in the sample; desalted proteins emerge from the column first and can be isolated by collecting discrete fractions of solution draining from the column tip. Dextran resin is stable in water, salt solutions, organic solvents and alkaline or acidic conditions. It has good rigidity and excellent flow properties. Dextran resin is heat-stable and can be autoclaved dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties.

Highlights:

  • Format: pre-packed gravity-flow (drip) columns

  • Resin type: porous, crosslinked, beaded dextran (50 to 150μm dia.)

  • Flow rate: approx. 1mL/min (relatively fast compared to other desalting resins)

  • Chemical stability: compatible with water, salt solutions, many organic solvents and alkaline or acidic conditions

  • Mechanical stability: medium rigidity compared to other gel-filtration resins

  • Heat stability: autoclavable dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties

Includes:

Pre-packed columns with top and bottom caps

Recommended for:

Removing salts from protein solutions; Removing phenol from nucleic acid preparations; Separating excess crosslinker from conjugate preparations; Removing excess derivatizing agents from modified proteins; Removing unreacted dye from fluorescent antibodies; Removing free radiolabel from labeled proteins; Exchanging one buffer for another