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Thermo Scientific™ Pierce™ Chemiluminescent Transcription Factor Assay Kits


Capture and measure NF-kappa B (NFkB) p50 or p65 transcription factors in 96-well plates using using ELISA-like plate assays and a SuperSignal ECL substrate.

Manufacturer: thermo scientific™  89859

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Catalog No. PI89859

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Description & Specifications

Specifications

Description Pierce NF-kappaB p65 Transcription Factor Assay Kit
Formulation Multi-component kit
Kit Contents 96-Well Assay Plates, 2 plates
Quantity 2 x 96 test kit
Sufficient For 192 assays with 10 to 25uL nuclear or whole cell protein extract

Thermo Scientific™ Pierce Chemiluminescent Transcription Factor Assay Kits are complete systems containing all the necessary components to measure NF kappa B p50 or p65 transcription factors in a 96-well microplate format.

These NF kappa B p50 and p65 Transcription Factor Assay Kits are complete systems containing all the necessary components to measure activated transcription factors in a 96-well microplate format. Kits contain two streptavidin-coated 96-well plates with the bound biotinylated-consensus sequence for the respective factor. The consensus duplexes bind only the active forms of the target transcription factors, diminishing nonspecific binding. This provides greater signal-to-noise ratios than a traditional ELISA. The captured active transcription factor is then detected with specific primary antibody (NFkB p50 or NFkB p65) and a secondary HRP conjugated antibody. Finally, a chemiluminescent substrate is then added to the wells, allowing quantitative measurement with a luminometer or CCD camera.

Highlights:

  • Sensitivity – chemiluminescent substrate yields results 20 to 140 times more sensitive than the colorimetric methods with a broader dynamic range

  • Speed – format allows researcher to perform multiple tests in three to four hours

  • Reliability – each kit contains a positive control lysate, as well as both wild-type and mutant competitor duplexes, ensuring reliable results with a gauge for specificity

  • High-throughput compatibility – 96-well format increases sample throughput over traditional EMSA

  • Convenience – kits are complete and contain all the reagents for two 96-well assay plates

  • Versatility – chemiluminescent signal is detectable using a luminometer or CCD Camera

Includes:

Two 96-well plates; 5X binding buffer; 20X poly dI-dC; Primary antibody; Antibody dilution buffer; HRP-conjugated secondary antibody; Luminol/enhancer solution; Stable peroxide solution; Two plate sealers; 10X wash buffer; Wild type and mutant duplex; Activated HeLa cell nuclear extract (positive control)