Thermo Scientific™ Pierce™ Chemiluminescent Transcription Factor Assay Kits
Capture and measure NF-kappa B (NFkB) p50 or p65 transcription factors in 96-well plates using using ELISA-like plate assays and a SuperSignal ECL substrate.
Manufacturer: Thermo Scientific™ PI89858
Thermo Scientific™ Pierce Chemiluminescent Transcription Factor Assay Kits are complete systems containing all the necessary components to measure NF kappa B p50 or p65 transcription factors in a 96-well microplate format.These NF kappa B p50 and p65 Transcription Factor Assay Kits are complete systems containing all the necessary components to measure activated transcription factors in a 96-well microplate format. Kits contain two streptavidin-coated 96-well plates with the bound biotinylated-consensus sequence for the respective factor. The consensus duplexes bind only the active forms of the target transcription factors, diminishing nonspecific binding. This provides greater signal-to-noise ratios than a traditional ELISA. The captured active transcription factor is then detected with specific primary antibody (NFkB p50 or NFkB p65) and a secondary HRP conjugated antibody. Finally, a chemiluminescent substrate is then added to the wells, allowing quantitative measurement with a luminometer or CCD camera.
Sensitivity – chemiluminescent substrate yields results 20 to 140 times more sensitive than the colorimetric methods with a broader dynamic range
Speed – format allows researcher to perform multiple tests in three to four hours
Reliability – each kit contains a positive control lysate, as well as both wild-type and mutant competitor duplexes, ensuring reliable results with a gauge for specificity
High-throughput compatibility – 96-well format increases sample throughput over traditional EMSA
Convenience – kits are complete and contain all the reagents for two 96-well assay plates
Versatility – chemiluminescent signal is detectable using a luminometer or CCD Camera
Two 96-well plates; 5X binding buffer; 20X poly dI-dC; Primary antibody; Antibody dilution buffer; HRP-conjugated secondary antibody; Luminol/enhancer solution; Stable peroxide solution; Two plate sealers; 10X wash buffer; Wild type and mutant duplex; Activated HeLa cell nuclear extract (positive control)
|2 x 96 test kit|
|192 assays with 10 to 25uL nuclear or whole cell protein extract|
|96-Well Assay Plates, 2 plates|
|Pierce NF-kappaB p50 Transcription Factor Assay Kit|