Thermo Scientific™ Pierce™ c-Myc Tag IP/Co-IP Kit
Anti-c-Myc antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving c-Myc-tagged fusion proteins.
Manufacturer: Thermo Scientific™ PI23620
Thermo Scientific™ Pierce c-Myc Tag IP/Co-IP Kit provides the affinity resin and other reagents necessary to easily perform immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments using a c-Myc-tagged protein as the bait.The c-Myc peptide (EQKLISEEDL) has become a popular fusion tag for mammalian recombinant protein expression, and this kit facilitates immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments involving c-Myc-tagged proteins. The kit includes crosslinked beaded agarose to which a highly specific anti-c-Myc antibody is covalently immobilized. Upon incubation with a sample containing the tagged fusion protein, interaction complexes involving the c-Myc-tagged bait protein are captured on the agarose beads. After simple washing steps, the specific protein interaction complex is easily eluted from the resin in the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis. The kit includes the prepared agarose affinity resin, buffers, microcentrifuge spin columns, a positive control and easy-to-follow instructions.
Specific – the immobilized anti-c-Myc monoclonal antibody binds the c-Myc epitope tag with high specificity, providing high yield immunoprecipitation products and clean Western blot detection
High capacity – excellent results with as little as 1μg of anti-c-Myc antibody in IP mode with the positive control lysate
Rigorous – kit includes a positive control lysate that contains over-expressed GST-c-Myc to validate the reagents and specific protocol, and the instructions provide tips for planning other controls
Robust – the affinity system is compatible with IP or Co-IP from various cell lysates and physiological (non-denaturing) buffer systems; choose kits with or without mammalian cell lysis buffer
Convenient and easy – complete kit includes all necessary reagents, convenient spin columns, and easy-to-follow instructions
M-PER™ Mammalian Protein Extraction Reagent (Part No. 23625 only), anti-c-Myc agarose resin, TBS pack, elution buffer, lane marker nonreducing sample buffer, positive control lysate, microcentrifuge spin columns and collection tubes
|Pierce c-Myc-Tag IP/Co-IP Kit|
|Positive Control Lysate (c-Myc-tagged GST), 0.5mL, Microcentrifuge Collection Tubes, 100 tubes, Pierce Spin Columns and Accessories, 27 columns, TBS Pack (makes 500mL), 1 pack, Anti-c-Myc Agarose Resin, 250uL, Elution Buffer (pH 2.8), 50mL, Non-Reducing Sample Buffer (5X), 5mL|
|25 IP reactions each using 5uL resin (5ug anti-c-Myc antibody)|
|Multi-component kit with anti-c-Myc agarose resin|
|25 rxn kit|