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Thermo Scientific™ Pierce™ BS3 (Sulfo-DSS) Crosslinker

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Manufacturer:  Thermo Scientific™ PI21580

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Catalog No. PI21580


 

Specifications

Description

Thermo Scientific™ Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Highlights:

  • Crosslink cell-surface proteins before cell lysis and immunoprecipitation
  • Identify receptor-ligand interactions by chemical crosslinking
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Identify near-neighbor protein interactions
  • Protein crosslinking to create bioconjugates via single-step reactions
  • Immobilize proteins onto amine-coated surfaces
Description & Specifications

Specifications

Amine-to-Amine
Pierce BS3 (Sulfo-DSS)
No
No
87mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
Yes
No
Bis(sulfosuccinimidyl) suberate; powder solid
572.43
50mg
11.4Å