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Thermo Scientific™ Pierce™ Biotin-Conjugated Proteins and Molecules

Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).

$102.38 - $489.30

Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

PI22030

 
thermo scientific™
PI22030
Pierce Biotin-Fluorescein Conjugate 5mg Each for $489.30

PI29129

 
thermo scientific™
29129
Pierce Biotin 1g Pack for $102.38

PI29130

 
thermo scientific™
29130
Pierce Bovine Serum Albumin, Biotinylated 25mg Pack for $255.15

PI29139

 
thermo scientific™
PI29139
Pierce Horseradish Peroxidase, Biotinylated 5mg Pack for $236.25

PI29339

 
thermo scientific™
PI29339
Pierce Alkaline Phosphatase, Biotinylated 1mg Pack for $369.60
Description & Specifications

Thermo Scientific™ Pierce Biotinylated Proteins include biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and fluorescein (B-FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method. Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.

Highlights:

  • Biotinylated enzymes – biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining

  • Biotinylated FITC – amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)

  • Biotinylated controls – use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods