Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification
Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.
Manufacturer: Thermo Scientific™ PI21027
Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.
The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.
Three optimized binding buffers – for Protein A, Protein G or Protein A/G
Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)
Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose
Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
IgG Elution Buffer: pH 2.8; amine-based
Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)
Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide
Kits contain both binding and elution buffers
|Pierce Gentle Ag/Ab Elution Buffer, pH 6.6|
|Gentle Ag/Ab Elution Buffer; 500mL|
|Proprietary, high-molarity buffer (pH 6.55)|
|Near-neutral elution of IgG|