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Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification


Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.

Manufacturer: thermo scientific™  PI21011

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Catalog No. PI21011

  • / Each
 

Description & Specifications

Specifications

Description Pierce Protein G IgG Binding Buffer
Formulation Proprietary buffer (pH 5) with sodium azide
Quantity 3.75L
Sufficient For Maximum binding of IgG to Protein G resin
Format Liquid
Target IgG
Item Description IgG Binding Buffer for Protein G; 3.75L

Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.

Highlights:

  • Three optimized binding buffers – for Protein A, Protein G or Protein A/G

  • Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)

  • Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose

Item-Specific Features:

  • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA

  • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA

  • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide

  • IgG Elution Buffer: pH 2.8; amine-based

  • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide

  • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

  • Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide

Includes:

Kits contain both binding and elution buffers