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Avidin Protein and Conjugates, Invitrogen™


Perform Western blot, ELISA or other biotin-based detection methods using these purified and HRP-, AP- or FITC-labeled avidin molecules.

Manufacturer: pierce biotechnology inc  29994

Catalog No. PI29994

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Description & Specifications

Specifications

Description Pierce Avidin, Horseradish Peroxidase Conjugated
Formulation Lyophilized powder from protein in buffered saline solution
Quantity 5mg

Thermo Scientific™ Pierce Avidin and Conjugates include purified avidin protein from hen egg whites, plus avidin-HRP, avidin-AP and other conjugated forms for use in biotin-based detection methods.

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

Highlights:

  • Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10

  • Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin

  • Affordable – significantly less expensive than recombinant streptavidin

  • ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)

  • Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays