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Thermo Scientific™ Pierce™ Amino Acids for SILAC Labeling


Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Manufacturer: thermo scientific™  88434

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Catalog No. PI88434

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Description & Specifications

Specifications

Description L-Arginine-HCl, 13C6, 15N4
Formulation Lyophilized powder of L-Arginine-HCl with at least 98% 13C6, 15N4
For Use With (Application) SILAC experiments (stable isotope labeling with amino acids in cell culture)
Quantity 500mg
Sufficient For Media for 10 SILAC experiments

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases

  • Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment

  • High-quality supplements – heavy amino acids with >99% isotope purity