Thermo Scientific™ Pierce™ Amino Acids for SILAC Labeling
Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.
Manufacturer: Thermo Scientific™ 89989
Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.
Efficient – 100% label incorporation into proteins of living cells
Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases
Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment
High-quality supplements – heavy amino acids with >99% isotope purity
|SILAC experiments (stable isotope labeling with amino acids in cell culture)|
|Media for one SILAC experiment|
|Lyophilized powder of L-Arginine-HCl (at least 98% pure)|
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