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Thermo Scientific™ Pierce™ AKT In-Cell ELISA Kit

Measure relative total and phosphorylated Akt protein kinase levels using Akt antibodies detected by fluorescence or TMB substrate in these whole-cell microplate assays.

Manufacturer:  Thermo Scientific™ 62220

 View more versions of this product

Catalog No. PI62220



Thermo Scientific™ Pierce AKT In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure total and phosphorylated Akt family of serine/threonine-specific protein kinases (phospho-AKT) in whole cells.

Pierce AKT In-Cell ELISA Kits enable the relative quantitation of total and phosphorylated Akt family of serine/threonine-specific protein kinases (AKT1, AKT2 and AKT3) in whole cells using a 96- or 384-well microplate format. The complete kits include a mouse monoclonal anti-AKT antibody, rabbit monoclonal anti-phospho-(Ser473) AKT antibody, detection reagents and all buffers required for either colorimetric or near infrared detection.

The Colorimetric In-cell ELISA Kit facilitates comparison of multiple target protein levels from different wells using target-specific antibodies, HRP-conjugated detection reagents and TMB substrate. Results across wells are then normalized to cell number based on whole-cell staining with Janus Green.

The Near Infrared In-Cell ELISA Kit allows simultaneous detection of two different target proteins within the same well, eliminating variability caused by differences in cell plating. With the near IR system, protein levels are determined by using target-specific primary antibodies and DyLight™ Fluor-Conjugated Secondary Antibodies. The near IR detection method eliminates issues with background fluorescence, providing a high signal-to-noise ratio and making these fluorescent multiplex experiments both sensitive and robust.


  • In-cell ELISA – target-specific detection directly from plated whole cells; no cell lysis, protein extraction or special sample preparation required

  • Quantitative and specific – system provides accurate measurement of relative protein expression levels in treated vs. nontreated cells

  • Better than Western blotting – In-cell ELISA directly yields quantitative results and provides higher throughput than Western blotting techniques

  • Less expensive – costs less (one well vs. one lane) than Western blotting methods of protein expression and activation analysis

  • Multiplex-capable – replicate wells or different fluors enable multiple ELISA targets to be measured relative to the primary targets supplied with the kit

  • Two kit formats – choose the colorimetric kit for use with standard microplate readers; choose the near infrared fluorescent detection kit for use with IR imaging systems


Anti-Phospho AKT (S473) Antibody, Anti-AKT Antibody, secondary antibodies, buffers, detergents and plate seals



Pierce AKT Near Infrared In-Cell ELISA Kit
Thin Plate Seal Assembly, 8 ea, Surfact-Amps X-100 (10% Detergent), 10mL, Surfact-Amps 20 (10% Detergent), 10mL, DyLight 800 Goat Anti-Rabbit IgG (H+L), 55uL, DyLight 680 Goat Anti-Mouse IgG (H+L), 55uL, Blocking Buffer, 100mL, 20X Tris Buffered Saline, 50mL
96 microplate wells of overnight-cultured cells
Multi-component kit with fluorescent conjugates
96 test kit