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Thermo Scientific™ Pierce™ 10X Methanol-free Western Blot Transfer Buffer

A methanol-free concentrated solution to easily and quickly prepare MeOH-free Western blot transfer buffer for tank-based or other wet gel-to-membrane transfer methods.

Manufacturer: thermo scientific™  35045

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Catalog No. PI35045


Description & Specifications


Description Pierce 10X Western Blot Transfer Buffer, Methanol-free
Formulation Proprietary aqueous buffer
Quantity 500mL
Sufficient For Preparing 5L of 1X buffer
Applications Electrophoresis
Composition Proprietary
Item Description 500mL

Thermo Scientific Pierce 10X Western Blot Transfer Buffer, Methanol-free is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) Western blotting transfer appratus.

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Western Blot Transfer Buffer is ready to use upon dilution to 1X with water; it does not contain methanol and the addition of methanol is not necessary for use in electrophoresis.


  • Transfer Buffer diluted 10-fold in water, the solution is ready to use for electrophoresis (i.e., wet tank transfer from mini gels)

  • Easy to use no packets to open, no powder to dissolve, and no methanol required

  • Increased accuracy eliminates the possibility of powder remaining in a packet

  • Saves time 10X concentration eliminates time spent waiting for powder to dissolve

  • Saves space storage as concentrated stock minimizes bench space needed for solutions

Recommended for:

Wet (tank) Western blot transfer of proteins to a membrane