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Phycoerythrin Mouse anti-Tag, Biotin, Clone: eBioPE-DLF, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS13412082

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Catalog No. 501129375



Description

Description

Description: The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested. Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Anti-RPE (R-Phycoerythrin) antibodies can be used to bind conjugated primary antibody without decreasing fluorescent intensity of the RPE fluorochrome. This method is used for separation of cells labeled with RPE-conjugated antibodies.
Specifications

Specifications

Phycoerythrin
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
R-phycoerythrin; R-PE; RPE; PE
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
Tag
Flow Cytometry
eBioPE-DLF
Biotin
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Documents

For Research Use Only.