Please login to your online account to display your discounted pricing

Thermo Scientific™ Phusion Hot Start High-Fidelity DNA Polymerase

Increase the specificity of PCR amplification by preventing non-specific extension with this highly accurate DNA polymerase, now available with a hot start modification.

$169.05 - $674.10

Specifications

Description Phusion™ Hot Start High-Fidelity DNA Polymerase
For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes Phusion Hot Start High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

F540L

 
thermo scientific™
F-540L
500U (2U/µL) Each for $674.10

F540S

 
thermo scientific™
F-540S
100U (2U/µL) Each for $169.05
Description & Specifications

Specifications

Description Phusion™ Hot Start High-Fidelity DNA Polymerase
For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes Phusion Hot Start High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.

Phusion High-Fidelity DNA Polymerase with a dsDNA-binding domain is now available with a hot start modification. The new Affibody-based inactivation method of Phusion DNA Polymerase increases the specificity of PCR amplification by preventing non-specific extension of DNA substrate at ambient temperatures prior to the first cycle of PCR. In addition to the extreme fidelity, speed and robustness, Phusion Hot Start DNA Polymerase now also delivers extreme specificity to PCR experiments. Protocols optimized for the original Phusion High-Fidelity DNA Polymerase can be directly applied to Phusion Hot Start DNA Polymerase reactions.

General information about Thermo Scientific Phusion DNA Polymerases, their advantages and applications can be found on Phusion DNA Polymerase products page.

Storage buffer

20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50% glycerol.

Storage

Recommended storage temperature -20°C.