Thermo Scientific™ Phusion Hot Start High-Fidelity DNA Polymerase
Increase the specificity of PCR amplification by preventing non-specific extension with this highly accurate DNA polymerase, now available with a hot start modification.
Manufacturer: Thermo Scientific™ F540L
Phusion High-Fidelity DNA Polymerase with a dsDNA-binding domain is now available with a hot start modification. The new Affibody-based inactivation method of Phusion DNA Polymerase increases the specificity of PCR amplification by preventing non-specific extension of DNA substrate at ambient temperatures prior to the first cycle of PCR. In addition to the extreme fidelity, speed and robustness, Phusion Hot Start DNA Polymerase now also delivers extreme specificity to PCR experiments. Protocols optimized for the original Phusion High-Fidelity DNA Polymerase can be directly applied to Phusion Hot Start DNA Polymerase reactions.
General information about Thermo Scientific Phusion DNA Polymerases, their advantages and applications can be found on Phusion DNA Polymerase products page.
20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50% glycerol.
Recommended storage temperature -20°C.
|Phusion™ Hot Start High-Fidelity DNA Polymerase|
|Phusion Hot Start High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.|
|Recommended storage temperature -20°C. Stable for one year from the assay date.|
|Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray|