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Thermo Scientific™ Phusion High-Fidelity DNA Polymerase


Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Manufacturer: thermo scientific™  F-553S

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 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. F553S

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Description & Specifications

Specifications

For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS
Quantity 50 x 50µL Rxns (125 x 20µL Rxns)
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the packaging date.

Phusion DNA Polymerases set a gold standard for PCR performance. They offer a combination of characteristics that no other enzyme can match. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion DNA Polymerase is the most accurate thermostable polymerase available. This feature makes Phusion DNA Polymerases superior choice for cloning and other applications requiring high fidelity. The unique structure of the enzyme enables short protocol times, abundant yields and robust performance, even in the presence of PCR inhibitors. For the researcher, that translates to ease-of-use and convenience. In addition, Phusion DNA Polymerases also produce higher yields with lower enzyme amounts than other DNA polymerases.

New Phusion Green format is a combination of Phusion DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with excelent performance of Phusion DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Phusion High-Fidelity PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control template and primers.

Highlights

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with Green Buffer