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Phospho-ZAP-70/SYK (Y319/Y352) Mouse anti-Human, Mouse, PerCP-eFluor 710, Clone: n3kobu5, eBioscience™

Mouse Monoclonal Antibody

$201.55 - $520.55

Specifications

Antigen Phospho-ZAP-70/SYK (Y319/Y352)
Clone n3kobu5
Host Species Mouse
Gene Alias Zeta chain-associated protein of 70 kD/Spleen tyrosine kinase
Species Reactivity Human, Mouse
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501123925

 
affymetrix
46900641
25 tests Each for $201.55

501129064

 
affymetrix
46-9006-42
100 tests Each for $520.55
Description & Specifications

Specifications

Antigen Phospho-ZAP-70/SYK (Y319/Y352)
Clone n3kobu5
Host Species Mouse
Gene Alias Zeta chain-associated protein of 70 kD/Spleen tyrosine kinase
Species Reactivity Human, Mouse
Applications Flow Cytometry (Intracellular Staining)
Regulatory Status RUO
Conjugate PerCP-eFluor 710
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 5μL (0.03μg)/test

This n3kobu5 monoclonal antibody recognizes human and mouse zeta chain-associated protein of 70 kD (also known as ZAP-70) and spleen tyrosine kinase (also known as SYK) when phosphorylated on Y319 and Y352, respectively. ZAP-70 and SYK are members of the SYK protein tyrosine kinase (PTK) family that are phosphorylated and activated by Src family PTKs. ZAP-70/SYK Y319/Y352 are located in the so-called interdomain of ZAP-70/SYK (between the N-terminal dual SH2 domains and the C-terminal kinase domain).

Phosphorylation of ZAP-70 Y319 by Lck is necessary for T cell receptor (TCR)-dependent association of ZAP-70 with Lck and phospholipase C gamma and subsequent activation of calcium-dependent and Ras signaling cascades. SYK Y352 phosphorylation by Fyn/Lyn is critical for propagation of B cell receptor (BCR) signaling and for B cell development.

Specificity of this n3kobu5 clone was determined by ELISA, flow cytometry, and western blotting.