Please login to your online account to display your discounted pricing

Phospho-H2A.x (S139) Mouse anti-Human, Mouse, eFluor 660, Clone: CR55T33, eBioscience™

Mouse Monoclonal Antibody

$187.05 - $448.05

Specifications

Antigen Phospho-H2A.x (S139)
Clone CR55T33
Host Species Mouse
Gene Alias Histone H2AX
Species Reactivity Human, Mouse
View More Specs

 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501124569

 
affymetrix
50986541
25 tests Each for $187.05

501124570

 
affymetrix
50986542
100 tests Each for $448.05
Description & Specifications

Specifications

Antigen Phospho-H2A.x (S139)
Clone CR55T33
Host Species Mouse
Gene Alias Histone H2AX
Species Reactivity Human, Mouse
Applications Flow Cytometry (Intracellular Staining)
Applications Immunocytochemistry
Regulatory Status RUO
Conjugate eFluor 660
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL; 5μL (0.125μg)/test

The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK)) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair.

The monoclonal antibody CR55T33 recognizes a single band of approximately 15kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.