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Phospho-H2A.x (S139) Mouse anti-Human, Mouse, Alexa Fluor 488, Clone: CR55T33, eBioscience™

Mouse Monoclonal Antibody

$187.05 - $433.55

Specifications

Antigen Phospho-H2A.x (S139)
Clone CR55T33
Host Species Mouse
Gene Alias Histone H2AX
Species Reactivity Human, Mouse
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501124658

 
affymetrix
53986580
25μg Each for $187.05

501124659

 
affymetrix
53986582
100μg Each for $433.55
Description & Specifications

Specifications

Antigen Phospho-H2A.x (S139)
Clone CR55T33
Host Species Mouse
Gene Alias Histone H2AX
Species Reactivity Human, Mouse
Applications Immunocytochemistry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Microscopy
Regulatory Status RUO
Conjugate Alexa Fluor 488
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.5mg/mL

The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair.

The monoclonal antibody CR55T33 recognizes a single band of approximately 15kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.