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Phospho-Histone H2A.X (Ser139) Mouse anti-Human, Mouse, Alexa Fluor® 488, Clone: CR55T33, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS53986582

Catalog No. 501124659



Description

Description

Description: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryo

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
Specifications

Specifications

Phospho-Histone H2A.X (Ser139)
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P27661, P16104
Histone H2A type 2-C, H2A 2C, H2AFQ, HIST2H2AC, Histone H2A/q
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
CR55T33
Alexa Fluor® 488
H2AFX
Liquid
H2AFX
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
15270, 3014
Documents

For Research Use Only.